Coding
BMP2

Part:BBa_K415500:Design

Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-24)


BMP2 L1L2 MammoBlock Entry Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1218
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1218
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1218
    Illegal AgeI site found at 11
    Illegal AgeI site found at 313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 218


Design Notes

The plasmid was assembled by using primers with attB recombination sites to PCR a BMP2 insert with attB sites on either side. This construct was inserted into a P1P2 donor vector using the Gateway recombination system to yield an L1L2 MammoBlock entry vector.


Source

NCBI Link: http://www.ncbi.nlm.nih.gov/gene/650

cDNA for human homolog obtained from OpenBiosystems.

References